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Schematic diagram of the plasmid pCot1i used for RNAi-mediated gene silencing of Trcot1 in T. reesei. The key elements are as following: pdc1p, the pdc1 promoter; eno1p, the eno1 promoter; cot1, the cot1 gene fragment; pyr4, the expressing cassette for the pyr4 gene; ampr, the ampicillin resistance gene; ori, the plasmid replication origin motif.
