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. 2020 Nov 17;30(11):1651–1658. doi: 10.4014/jmb.2011.11003

Fig. 3. ZIKV NS2A and NS4A impeded activation of the NF-κB promoter by IRF FL, IRF3 1-390, and IRF3-5D.

Fig. 3

Various plasmids encoding individual ZIKV protein, NF-κB-luc, β-Gal, and IRF3 FL (A), IRF3 1-390 (B), IRF3-5D (C) were co-transfected to HEK293T cells. After 24 hours, the lysed cells were collected for luciferase assay (top panel) and western blotting (bottom panel) using anti-HA antibody (for IRF3 FL, IRF3 1-390, and IRF3-5D), anti-FLAG antibody (for ZIKV proteins), and anti-GAPDH antibody (for human GAPDH). Representative data from two independent experiments were presented. Student’s two-tailed t-test were applied for statistical significance, **p < 0.01, *p < 0.05. The sizes of the proteins are presented on the left (kDa) and arrows indicate the expected proteins.