Fig. 2.

Inhibitory effects of PEE on the protein production level of specific transcription factors in 3T3-L1 preadipocytes after 8-day differentiation. The protein production levels as values relative to β-actin of (A) adipogenesis (PPARγ, C/EBPα, and SREBP-1c) and (B) lipogenesis (FAS and ACC) transcription factors were measured using western blot analysis. Each value is expressed as the mean ± SD. CON: undifferentiated preadipocyte; MDI: differentiated adipocyte; 50: adipocyte was treated with 50 μg/ml of PEE; 100: adipocyte was treated with 100 μg/ml of PEE; 150: adipocyte was treated with 150 μg/ml of PEE; PPAR γ, peroxisome proliferator-activated receptor gamma; C/EBPα, CCAAT/enhancerbinding protein alpha; SREBP-1c, sterol regulatory element binding protein-1c; FAS, fatty acid synthase; ACC, acetyl-CoA carboxylase. Data represent the means ± SD in triplicate. ^a-e: Means the different letters on the bars are significantly different at p < 0.05.