Fig. 6.

Detection of polarized activation of macrophages in the lungs of hDPP4-Tg mice infected with MERS-CoV. Total RNA was extracted from lung tissues of hDPP4-Tg mice 4 and 6 days after infection with MERS-CoV and processed to evaluate changes in pulmonary macrophage polarization status by qRT-PCR. The relative expression of genes for (A) macrophage activation and (B) M2 surface markers, together with that of the internal control gene, β-actin, was analyzed by qRT-PCR in duplicate. Expression levels relative to those of the non-treated controls are shown as means ± SD. *p < 0.05 and **p < 0.01.