. 2020 May 21;30(8):1149–1155. doi: 10.4014/jmb.2003.03010

Table 2.

Perennial ryegrass, red clover and blue alfalfa silage hygienic quality after 96 days of fermentation.

Silage samples	DM ± SD, %	pH ± SD	Total count of aerobic bacteria ± SD, log₁₀ CFU/g	Count of yeast ± SD, log₁₀ CFU/g	Count of molds ± SD, log₁₀CFU/g	Total count of mesophilic lactic acid bacteria ± SD, log₁₀ CFU/g
Perennial ryegrass C	20.66 ± 1.52^b,c,d,e,f,g	6.11 ± 0.01^b,c,d,e,f,,g	2.36 ± 0.17	1.79 ± 0.02^b,c,d,e,f,g	0.84 ± 0.03^b,c,d,e,f,g	1.91 ± 0.01^b,c,e,g
Perennial ryegrass O-AE	32.66 ± 4.50^a,f	6.68 ± 0.02^a,c,d,e,f,g	2.48 ± 0.65	0.00 ± 0.00^a,c,d,f,g	0.00 ± 0.00^a,c,f,g	1.74 ± 0.02^a,c,d,f,g
Perennial ryegrass O-EE	33.00 ± 1.00^a,f	4.82 ± 0.02^a,b,d,e,f,g	2.64 ± 0.30	1.45 ± 0.04^a,b,d,e,f,g	0.76 ± 0.02^a,b,d,e,f,g	2.04 ± 0.00^a,b,d,e,f,g
Perennial ryegrass T-AE	30.66 ± 0.57^a,f,g	5.05 ± 0.02^a,b,c,e,f,g	2.81 ± 0.04	1.14 ± 0.01^a,b,c,e,g	0.00 ± 0.00^a,c,f,g	1.88 ± 0.01^b,c,e,g
Perennial ryegrass T-EE	32.00 ± 2.64^a,f	4.35 ± 0.01^a,b,c,d,f,g	2.49 ± 0.04	0.00 ± 0.00^a,c,d,f,g	0.00 ± 0.00^a,c,f,g	1.74 ± 0.03^a,c,d,f,g
Perennial ryegrass X	37.00 ± 1.00^a,b,c,d,e	4.47 ± 0.02^a,b,c,d,e	2.49 ± 0.25	1.17 ± 0.04^a,b,c,e,g	0.47 ± 0.05^a,b,c,d,e,g	1.88 ± 0.02^b,c,e,g
Perennial ryegrass Y	35.66 ± 0.57^a,d	4.48 ± 0.02^a,b,c,d,e	2.30 ± 0.01	0.60 ± 0.03^a,b,c,d,e,f	1.08 ± 0.03^a,b,c,d,e,f	1.80 ± 0.01^a,b,c,d,e,f

Red clover C	29.66 ± 1.52^y,k,l,m	4.36 ± 0.02^i,y,j,k,l,m	2.37 ± 0.10^i,j,k,l,m	1.92 ± 0.02^i,y,j,k,l	0.63 ± 0.04^i,j,k,l,m	2.35 ± 0.01^i,y,j,k,l,m
Red clover O-AE	30.33 ± 1.52^y,j,k,l,m	4.64 ± 0.01^h,y,j,k,l,m	2.00 ± 0.01^h,y,j,k,l	0.95 ± 0.03^h,y,j,k,l,m	0.00 ± 0.00^h,y,k,m	1.92 ± 0.02^h,y,j,k,l,m
Red clover O-EE	38.00 ± 2.00^h,i,j,k,l	4.13 ± 0.04^h,i,j,k,l	2.33 ± 0.05^i,j,k,l,m	1.78 ± 0.04^h,i,j,k,l,m	0.60 ± 0.02^i,j,k,l,m	1.23 ± 0.01^h,i,j,k,l,m
Red clover T-AE	27.66 ± 1.52^i,y,k,l,m	5.05 ± 0.02^h,i,y,k,l,m	1.79 ± 0.02^h,i,y,k,l,m	1.56 ± 0.01^h,i,y,k,l,m	0.00 ± 0.00^h,y,k,m	1.77 ± 0.01^h,i,y,k,l,m
Red clover T-EE	56.66 ± 1.15^h,i,y,j,l,m	5.43 ± 0.03^h,i,y,j,l,m	1.55 ± 0.12^h,i,y,j,l,m	1.22 ± 0.04^h,i,y,j,l,m	0.77 ± 0.01^h,i,y,j,l	1.64 ± 0.02^h,i,y,j,l,m
Red clover X	53.66 ± 1.52^h,i,y,j,k,m	4.31 ± 0.01^h,i,y,j,k,m	2.14 ± 0.05^h,i,y,j,k,m	0.84 ± 0.06^h,i,y,j,k,m	0.00 ± 0.00^h,y,k,m	1.45 ± 0.03^h,i,y,j,k,m
Red clover Y	38.66 ± 0.57^h,i,j,k,l	4.08 ± 0.01^h,i,j,k,l	2.01 ± 0.01 ^h,y,j,k,l	1.86 ± 0.01^i,y,j,k,l	0.77 ± 0.02^h,i,y,j,l	1.41 ± 0.04^h,i,y,j,k,l

Blue alfalfa C	26.66 ± 1.52^o,p,r,s,t,u	4.90 ± 0.05^o,p,r,s,t,u	1.65 ± 0.09^o,r,s,t,u	0.59 ± 0.01^o,p,r,s,t,u	0.30 ± 0.01^o,p,r,s,t,u	1.84 ± 0.03^o,p,r,s,t,u
Blue alfalfa O-AE	55.66 ± 1.15^n,p,r,s,u	4.30 ± 0.02^n,p,r,s,t,u	1.26 ± 0.08^n,p,r,s,t,u	0.00 ± 0.00^n,r,s,u	0.00 ± 0.00^n,r,s	1.30 ± 0.01^n,p,r,s,t,u
Blue alfalfa O-EE	49.66 ± 1.15^n,o,r,s,t,u	4.42 ± 0.02^n,o,r,s,t,u	1.69 ± 0.04^o,r,s,t,u	0.00 ± 0.00^c,r,s,u	0.00 ± 0.00^n,r,s	1.36 ± 0.01^n,o,r,s,t,u
Blue alfalfa T-AE	36.66 ± 0.57^n,o,p,t,u	4.52 ± 0.02^n,o,p,s,t,u	1.87 ± 0.01^n,o,p,s,t,u	0.46 ±0.02^n,o,p,s,t,u	0.69 ± 0.02^n,o,p,s,t,u	2.05 ± 0.01^n,o,p,s,t,u
Blue alfalfa T-EE	36.33 ± 1.52^n,o,p,t,u	3.88 ± 0.01^n,o,p,r,t,u	2.00 ± 0.01^n,o,p,r,t	1.11 ± 0.01^n,o,p,r,t	1.14 ± 0.05^n,o,p,r,t,u	1.73 ± 0.03^n,o,p,r,t,u
Blue alfalfa X	53.00 ± 1.73^n,p,r,s,u	5.27 ± 0.02^n,o,p,r,s,u	1.49 ± 0.08^n,o,p,r,s,u	0.00 ± 0.00^n,r,s,u	0.00 ± 0.00^n,r,s	1.49 ± 0.02^n,o,p,r,s,u
Blue alfalfa Y	45.66 ± 2.88^n,o,p,r,s,t	3.67 ± 0.01^n,o,p,r,s,t	2.06 ± 0.03^n,o,p,r,u	1.11 ±0.07^n,o,p,r,t	0.00 ± 0.00^n,r,s	1.97 ± 0.01^n,o,p,r,s,t

^a_gMean values with different superscripts are significantly different in perennial ryegrass silage samples (p ≤ 0.05). ^h_mMean values with different superscripts are significantly different in red clover silage samples (p ≤ 0.05). ^n_uMean values with different superscripts are significantly different in blue alfalfa silage samples (p ≤ 0.05).

SD – standard deviation

C – control sample, O-AE – oregano aqueous extract, O-EE – oregano ethanol extract, T-AE – thyme aqueous extract, T-EE –thyme ethanol extract, X – commercial inoculant X, Y – commercial inoculant Y.