FIG. 2.

Slot blot for the detection of the transcript for gyrase. RNA was derived from the midexponential (m exp), late exponential (l exp), and postexponential (p exp) growth phases of ISP479C (agr⁺) (the wild type [WT]), DU5723 (spa mutant), DU1090 (hla mutant), and ISP546 (agr mutant) (A) and ISP479C (the WT strain) and the CF isolates 8A, 9A, 10A, 10B and 11A (1:1 and 1:5 dilutions) (B). Samples were hybridized with a digoxigenin-labeled gene probe specific for S. aureus gyr. c, control dilution (1:1).