Fig. 2.

Inhibitory effects of resolvin D5 on LPS-induced IL-6 and CCL5 production in THP-1 cells. THP-1 cells were pretreated with resolvin D5 for 1 h and then treated with LPS (1 μg/ml) for 24 h. The IL-6 and CCL5 mRNA levels were evaluated by RT-qPCR in a resolvin D5 concentration-dependent manner (A, B). The IL-6 and CCL5 protein levels were evaluated by ELISA in a resolvin D5 concentration-dependent manner (C, D). The data were obtained from three independent experiments and reported as the mean ± SD (n = 3). *p < 0.05 (LPS alone versus LPS plus resolvin D5), **p < 0.01 (LPS alone versus LPS plus resolvin D5), as calculated by one-way ANOVA.