Fig. 4.

Effect of Undaria pinnatifida fucoidan-rich extract (DSU02) on the proliferation of whole splenocytes, CD3+, CD4+, and CD8+ T cells, in CP-treated immunosuppressed mice. Mice were intraperitoneally injected with 80 mg/kg of cyclophosphamide (CP) and subsequently administered with either drinking water (DW), RG extract (10 mg/kg), or one of three different doses of Undaria pinnatifida fucoidan-rich extract (DSU02 50 mg/kg, 100 mg/kg, 150 mg/kg). Primary splenocytes were isolated from each animal and stimulated with Con A (1 μg/ml ). (A) The proliferation of splenocytes was measured by CCK-8 assay at 72 h after incubation. Single cell suspension of splenocytes from each animal was stained with CFSE and cultured for another 7 days. (B) Representative FACS plot of CD3+, CD4+, and CD8+ expression on T cells. (C) The specific proliferations of CD3+, CD4+, and CD8+ were analyzed using flow cytometry. Significant difference is shown: ** or * p < 0.05.