Fig. 5. Membrane permeabilization assay performed by fluorescence microscopy of Sporothrix spp. yeast cells incubated with SYTOX Green.

(A and A’) Control cells; (B and B’) cells treated with itraconazole (positive control); (C and C’) cells treated with Ag₃[PMo₁₂O₄₀]; (D and D’) cells treated with Ag₃[PW₁₂O₄₀], (E and E’) cells treated with Ag₆[SiW₁₀V₂O₄₀]; (F and F’) cells treated with Ag₄[SiW₁₂O₄₀]. All conditions were viewed by DIC and by fluorescence. Magnification, 630X.