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. 2022 Nov 23;13:1054968. doi: 10.3389/fimmu.2022.1054968

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Copyright © 2022 Nikolic, Suwandi, Wesselius, Laban, Joosten, Sonneveld, Mul, Aanstoot, Kaddis, Zwaginga and Roep

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of tolDC on regulatory T-cell subsets. Pseudotime analysis (diffusion map) shows differentiation trajectory within Treg cluster 6. (A) Treg subclusters as defined in Cytosplore ( Figure 5E ) are visualized in the diffusion map. Subclusters 6.1 and 6.2 are plotted together since both subclusters show a naïve phenotype. (B) Specified markers within the diffusion map. (C) Frequency in time of total Treg and Treg subclusters, shows significant increase of eTregs (subcluster 6.4) 1 month after tolDC treatment (*p=0.035).