Table 2.
Transcriptional changes of global regulators in livestock-associated MRSAST398 exposed to ammonia porcine serum or both.
| Gene transcription (log2FC) compared to unchallenged control samples | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Porcine serum | Ammonia | Porcine serum & ammonia | |||||||
| ID | Symbol | Product | AA length | 10 min | 60 min | 10 min | 60 min | 10 min | 60 min |
| SACH_00575 | arlS | signal transduction histidine kinase | 452 | −0.4 | −0.1 | −0.6 | −1.8 | −0.4 | −0.9 |
| SACH_00576 | arlR | response regulator receiver | 220 | −0.6 | −0.2 | −0.7 | −1.6 | −0.6 | −0.9 |
| SACH_00645 | srrB | integral membrane sensor signal transduction histidine kinase | 584 | 0.1 | 0.2 | −0.4 | −0.6 | 0.6 | 0.2 |
| SACH_00646 | srrA | winged helix family two component transcriptional regulator | 242 | −0.2 | 0.4 | −0.6 | −1.1 | 0.8 | 0.5 |
| SACH_00713 | rpoD | RNA polymerase sigma-70 factor RpoD (SigA) | 369 | 0.9 | 0.1 | 0.3 | −0.1 | 0.7 | 0.7 |
| SACH_00905 | rot | repressor of toxins (Rot) | 134 | −0.3 | −0.1 | 1.6 | 1.9 | 1.5 | 1.4 |
| SACH_00917 | sigS | RNA polymerase factor sigma-70 | 157 | 1.0 | 0.5 | −0.3 | −0.4 | −0.2 | 0.1 |
| SACH_01193 | agrB | accessory gene regulator B | 208 | −0.9 | −0.4 | −2.1 | −3.7 | −1.8 | −4.7 |
| SACH_01194 | agrD | staphylococcal accessory gene regulator protein D | 47 | 0.3 | 0.5 | −1.6 | −3.4 | −1.2 | −4.5 |
| SACH_01195 | agrC | accessory gene regulator protein C | 431 | −0.6 | −0.5 | −2.1 | −4.0 | −1.9 | −4.6 |
| SACH_01196 | agrA | autoinducer sensor protein response regulator protein | 239 | −0.9 | −0.5 | −2.5 | −3.7 | −2.1 | −4.2 |
| SACH_01284 | sigB | RNA polymerase sigma factor SigB | 257 | 0.4 | −0.3 | 0.4 | −1.3 | 1.3 | 0.3 |
| SACH_01524 | sarR | staphylococcal accessory regulator R SarR | 116 | 0.0 | 0.5 | −1.9 | −4.1 | −2.8 | −2.2 |
| SACH_02036 | sarS | staphylococcal accessory regulator A SarS | 251 | 0.3 | 0.4 | −1.0 | −0.9 | 0.0 | 0.6 |
| SACH_02532 | sigH | RNA polymerase factor sigma-70 | 190 | 0.7 | 0.2 | 1.2 | 0.4 | 0.9 | 0.9 |
| SACH_02614 | sarA | staphylococcal accessory regulator family protein | 125 | 0.9 | −0.5 | 1.5 | −0.4 | 1.1 | −0.1 |
| SACH_02686 | mgrA | MarR family transcriptional regulator | 148 | −0.4 | 0.1 | −1.4 | −2.7 | −1.4 | −0.5 |
| SACH_02705 | saeS | integral membrane sensor signal transduction histidine kinase | 352 | −0.3 | 0.7 | −1.6 | −3.3 | −2.3 | −2.6 |
| SACH_02706 | saeR | winged helix family two component transcriptional regulator | 229 | −0.1 | 1.0 | −1.5 | −3.1 | −2.1 | −2.4 |
Transcriptional changes after 10 min and 60 min of MRSA IMT38951 exposed to MH II supplemented with 10% porcine serum. 0.3 ammonia or both, respectively. ID, Sequence identifier; AA length, amino acid length.