Extended Data Table 1.
Cryo-EM data collection, model refinement, and validation statistics.
| (EMDB-26067) (PDB 7TQL) |
|
|---|---|
| Data collection and processing | |
| Magnification | 135,000 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 47.38 |
| Defocus range (μm) | −0.5/Å2 |
| Pixel size (Å) | 0.852 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 2M |
| Final particle images (no.) | 190K |
| Map resolution (Å) | 3.2 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3-5 |
| Refinement | |
| Initial model used (PDB code) | 5LZS |
| Model resolution (Å) | 3.6 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | 3.2-6 |
| Map sharpening B factor (Å2) | −89 |
| Model composition | |
| Non-hydrogen atoms | 80954 |
| Protein residues | 7241 |
| Ligands | 32 |
| B factors (Å2) | |
| Protein | 122 |
| Ligand | 132 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.002 |
| Bond angles (°) | 1.23 |
| Validation | |
| MolProbity score | 1.24 |
| Clashscore | 1.1 |
| Poor rotamers (%) | 0.25 |
| Ramachandran plot | |
| Favored (%) | 93.62 |
| Allowed (%) | 99.61 |
| Disallowed (%) | 0.39 |