KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| [6,6-2H2]glucose | Cambridge Isotope Laboratories | Cat# DLM-349-MPT-PK |
| [U-13C]glucose | Cambridge Isotope Laboratories | Cat# CLM-1396-MPT-PK |
| [U-13C]palmitate | Cambridge Isotope Laboratories | Cat# CLM-3943-MPT-PK |
| Regular insulin (human recombinant) | Lily USA | Humulin R U100 |
| Dextrose 20% IV solution | Hospira | Cat# 793519 |
| 75-g glucose solution | ThermoFisher SCIENTIFIC | Cat# TGP401223PA |
| Acetonitrile for LC/MS | Fisher chemical | Cat# A955–4; CAS# 75–05-8 |
| Methanol for LC/MS | Fisher chemical | Cat# A456–4; CAS# 67–56-1 |
| Water for LC/MS | Fisher chemical | Cat# W6–4; CAS# 7732–18-5 |
| 2-propanol for LC/MS | Fisher chemical | Cat# A461–4; CAS# 67–63-0 |
| Medronic acid | MilliporeSigma | Cat# 64255; CAS# 1984–15-2 |
| Ammonium bicarbonate | MilliporeSigma | Cat# 5.33005; CAS# 1066–33-7 |
| Ammonium hydroxide | Fluka | Cat# 44273; CAS# 1336–21-6 |
| Formic acid | Fisher chemical | Cat# A117–50; CAS# 64–18-6 |
| Ammonium formate | MilliporeSigma | Cat# 70221; CAS# 540–69-2 |
| Critical Commercial Assays | ||
| Glucose concentration | Yellow Springs Instruments | YSI 2300 Stat Plus |
| Insulin and C-peptide concentrations | Roche | Elecsys 2010 immunoassays |
| Triglyceride concentration | Roche | Cat# 20767107322 |
| High density lipoprotein concentration | Roche | Cat# 07528566190 |
| HbA1c – Hemolyzing agent | Roche | Cat# 04528182190 |
| HbA1c - Assay | Roche | Cat# 05336163190 |
| Adiponectin concentration | MilliporeSigma | Cat# HADP-61HK |
| PAI-1 concentration | MilliporeSigma | Cat# HNDG3MAG-36K |
| Deposited data | ||
| Skeletal muscle RNA-sequencing data | NCBI GEO database | Accession# GSE205891 |
| Software and Algorithms | ||
| Excel | Microsoft | N/A |
| SPSS version 27 | IBM | N/A |
| R | R-Project | N/A |
| edgeR R Bioconductor | Bioconductor | N/A |
| Ingenuity Pathway Analysis | Qiagen | N/A |
| EndNote Version X9.3.3 | Clarivate Analytics | N/A |
| Skyline | University of Washington | https://skyline.ms |
| Qualitative Analysis 10.0 | Agilent Technologies | N/A |
| Other | ||
| TRIzol Reagent | Invitrogen | Cat# 15596018 |
| RNeasy mini kit | Qiagen | Cat# 74106 |
| RNase-free DNase Set | Qiagen | Cat# 79254 |
| iHILIC®-(P) Classic, Guard Column | HILICON | Cat# 160.122.0520 |
| iHILIC®-(P) Classic, HILIC Column | HILICON | Cat# 160.102.0520 |
| Acquity UPLC® HSS T3 VanGuard Pre-Column | Waters | Cat# 186003540 |
| Acquity UPLC® HSS T3 Column | Waters | Cat# 186003976 |