Enhancement and TB values for StaPol-1 and SNAPol-1 in biological preparations. In vitro samples utilized DNP juice (d8-glycerol/D2O/H2O, 60/30/10, v/v/v) whereas in cell and lysate samples utilized completely deuterated DNP juice (d8-12C3 glycerol/D2O, 60/40, v/v). *Data taken from ref. 32. **Data taken from Beriashvili et al., submitted 2022.
| Polarizing agent | Sample | 13C εon/off | T B (s) |
|---|---|---|---|
| StaPol-1 | In vitro | 105 ± 14a/117 ± 0.9b | 5.47a |
| SNAPol-1* | In vitro | 110a | 4.35a |
| StaPol-1 | In cell | 50 ± 1.65b | 2.4/24.5a |
| SNAPol-1** | In cell | 55b | 1.4/25.5a |
| StaPol-1 | Lysate | 183 ± 4.99c,d | 1.2/5.6d |
| SNAPol-1 | Lysate | 68 ± 5.20c/150 ± 1.96d | 0.34/4.3d |
a
Carbonyl (170–180 ppm).
b
Aliphatic (0–70 ppm).
c
Aliphatic (50–60 ppm).
d
Aliphatic (41 ppm). See Materials and methods in ESI for details of the error analysis.