FIG. 1.

Antigen-specific T cells are induced in mice by immunization with a mixture of recombinant proteins and different adjuvants. The mice were immunized with formulations containing all three recombinant proteins and the adjuvants as indicated in the figure (see Materials and Methods). Single cell suspensions of spleens (A) and lymph nodes (B) of vaccinated mice were restimulated in vitro in the presence of rMBAP, rCP5, rGP63 or a freeze-thawed lysate of L. mexicana as indicated. Cell proliferation was assessed by measuring [³H]thymidine incorporation. Antigen-specific cell proliferation is expressed by stimulation indices calculated from the incorporated radioactivity in the presence of antigen divided by the activity incorporated in cells cultured in medium only (background 5 × 10³ to 2.6 × 10⁴ cpm/culture well). Data represent the arithmetic mean of triplicate determinations.