FIG. 2.
Efficacy of different vaccine formulations containing rMBAP, rCP5, and rGP63. C57BL/6 mice were injected twice at a weekly interval into the lower back with 200 μl of vaccine containing 2.5 μg of each protein mixed with the indicated adjuvants. One week after the boosting injection, 4 × 105 L. mexicana promastigotes were injected into the hind left footpad. Footpad swelling was measured every second week, and the data represent the mean increase in thickness compared to the uninfected foot of five mice per group. One animal in the groups treated only with QS-21, Detox, and C. parvum showed a tendency to heal at the end of the experiment. However, the averaged values include all mice per group. The plots are arranged in two graphs: lesion development in groups immunized with QS-21, Detox, or C. parvum (A) and in groups immunized with BCG, MPL, or IL-12 (B). PBS controls are shown in both graphs.