FIG. 3.
Protective efficacy of vaccines containing native antigens and IL-12 or Detox as adjuvants. C57BL/6 mice (five per group) were vaccinated with two doses of 2.5 μg each of CP, GP63, and MBAP mixed with IL-12 or Detox as described in the text. Immunized animals were infected with 4 × 105 L. mexicana promastigotes. Lesion development was monitored as outlined in Fig. 2. In the group treated with antigen (Ag) and Detox, two of five animals still had active lesions at the end of the experiment, while one of five mice showed a tendency to heal in the groups treated only with IL-12 or Detox or PBS-antigen (PBS-Ag). Error bars are shown for one group only for better clarity and correspond to the mean ± the standard deviation (SD), including all mice, and are representative for all groups.