Table 1. Sample identification used for flow cytometry assay of farm pig adipose tissue-derived mesenchymal stromal/stem cells.
Sample identification | |||||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |
Primary antibodies (µL) | - | CD31 (-) 2 | CD29 (+) 2 | CD90 (+) 5 | CD45 (-) 5 |
Secondary antibody Alexa Fluor 647 (µL) | - | - | 0.5 | 0.5 | - |
PBS (µL) | 100 | 98 | 97.5 | 94.5 | 95 |
Total volume (µL) | 100 | 100 | 100 | 100 | 100 |
Components and volumes used for each sample tested by flow cytometry assay. PBS: phosphate-buffered saline.