FIG. 2.

The effect of rEUO on in vitro transcription from the crp operon. The templates were empty vector without insert (EV); pLZ208C, containing the C. psittaci crp operon promoter from −404 to +12 (lanes 2 to 9); and pALR202, containing the C. trachomatis L2 plasmid antisense transcripts promoter (PT) from −498 to +6. The concentrations of EUO protein (lanes 2 to 5) and an irrelevant E. coli protein (lanes 6 to 9), purified from the same gel from which rEUO was purified, are shown above the lanes; no recombinant protein was present in the EV and PT controls. The position of the in vitro-generated crp and PT transcripts are indicated on the right side. Other bands represent transcription from unknown sites within the vector or the inserts. A DNA ladder, which was used to estimate the length of the transcripts, is shown on the left side.