FIG. 5.

Insert DNA fragments of representative clones enriched at rounds 5 and 9. The plasmids of 20 random clones picked after round 5 (top panel) or round 9 (bottom panel) were digested with HindIII and XbaI and fractionated by agarose gel electrophoresis. Lanes marked M represent linear DNA markers (in kilobases). ○, clones with an insert size of approximately 500 bp. DNA sequencing revealed that all of these clones contain the same insert, which was designated LZ9R8. #, clones with identical insert DNA fragments totaling about 1.2 kb. The insert of these clones was designated LZ9R17. ●, clones with identical inserts of 2.3 kb, which were designated LZ9R5. Migration of the linear vector (pBluescript [pBS]) is indicated on the right side.