Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | NCI-H226 (Mesothelioma, Male) | ATCC | CRL-5826 | |
| Cell line (Homo sapiens) | MSTO-211H (Mesothelioma,Male) | ATCC | CRL-2081 | |
| Cell line (Homo sapiens) | NCI-H2452 (Mesothelioma, Male) | ATCC | CRL-5946 | |
| Cell line (Homo sapiens) | MeT-5A (Mesotheliumcells) | ATCC | CRL-9444 | |
| Antibody | Anti-human YAP (Rabbit monoclonal) | Cell Signaling | Cat#14074 S | WB (1:1000) |
| Antibody | Anti-human pan-TEAD (Rabbit monoclonal) | Cell Signaling | Cat#13295 S | WB (1:1000) |
| Antibody | Anti-human TEAD4 (Mouse monoclonal) | Abcam | Cat#ab58310 | WB (1:1000) |
| Antibody | Anti-human b-actin (Mouse monoclonal) | Cell Signaling | Cat#3700 S | WB (1:2000) |
| Recombinant DNA reagent | Myc-TEAD4 (plasmid) | Addgene | RRID: Addgene_24638 | Overexpression of Myc-TEAD4 |
| Recombinant DNA reagent | TBS-mCherry vector (plasmid) |
Cancer Cell. 2020 Jan 13; 37(1): 104–122.e12 | Monitor TEAD transcriptional activity | |
| Commercial assay or kit | Dynabeads Co-Immunoprecipitation Kit | ThermoFisher Scientific | Cat#14321D | |
| Commercial assay or kit | SuperScript III First-Strand Synthesis System Kit | ThermoFisher Scientific | Cat#18080051 | |
| Chemical compound, drug | Palmitoyl alkyne-coenzyme A | Cayman chemical | Cat#15968 | |
| Chemical compound, drug | Alkyne palmitic acid | Click Chemistry Tools | Cat#1165–5 | |
| Software, algorithm | GraphPad9 | GraphPad | ||
| Other | TEAD luciferase reporter lentivirus | BPS Biosciences | Cat#79833 | Used to establish a stable cell line to monitor TEAD transcriptional activity; related to Figure 6—figure supplement 3 (a) |
| Other | IRDye 800CW Streptavidin | LI-COR | Cat# 926–32230 | WB (0.2 μg/mL) |