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. 2022 Nov 30;2022:5266627. doi: 10.1155/2022/5266627

Figure 1.

Figure 1

Exosomes derived from MDA-MB-231 and BT549 cells promoted the transition of HMFs to the CAF phenotype. (a) Exosome identification by TEM. (b) Size distribution of MDA-MB-231- and BT549-derived exosome diameters. (c) Detection of TSG101 and HSP70 protein expression by western blot. (d) Human mammary fibroblasts. (e) Exosome uptake assay observed HMFs taking in MDA-MB-231- and BT549-derived exosomes. (f) Immunofluorescence staining observed the expression of α-SMA and FAPα in HMFs uptaking MDA-MB-231 and BT549 cell-derived exosomes.