Figure 5.

Exosomal miR-425-5p stimulated HMF switch to CAF phenotype. In miR-425-5p transfected CAFs (HMFs uptaking exosomes from MDA-MB-231 cells), cell proliferation (a), invasion (b), migration (c), the expression of α-SMA and FAPα (d), CXCL1, TGFβ1, and IL6 (e), P21, P27, and Ki67 (f), and vimentin, E-cadherin, N-cadherin, α-catenin, fibronectin, and MMP2 (g) were detected by RT-PCR and western blot assays. Experimental data presented as means ± standard deviation. Experiment was repeated 3 times. ^∗∗P < 0.01 compared with the MDA-MB-231 group. ^##P < 0.01 compared with the CAF miR-NC group.