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. 2022 Dec 5;54(12):1919–1932. doi: 10.1038/s41588-022-01223-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, Schematics for endogenously tagging CTCF/cohesin with AID in the HaloTag-YY1 cell line (YY1-HT, clone YN11) and western blots of CTCF, RAD21 and β-actin. b, Airyscan-resolved, live-cell confocal imaging for HaloTag-YY1 stained with 500 nM TMR Halo ligand in CTCF- or RAD21-depleted cells (n = 6 for each depletion). Scale bar, 1 μm. c, PALM imaging for YY1 (n = 13 for each depletion). Color maps color the signal ranging from 0 to 40. Scale bar, 1 μm. d, Stacked bar graph showing the fractions of bound, slow- and fast-diffusing YY1 in the untreated and IAA-treated cells, obtained by SASPT analysis (n = 8 cells examined over three independent experiments). The statistical test used was the two-sided Student’s t-test. NS, not significant. Error bars indicate mean ± s.d. e, FRAP analysis of YY1 in the control, CTCF-depleted or RAD21-depleted cells. f, Heatmaps (left) and histogram profiles (right) of differential ChIP-seq signals for YY1 after CTCF, RAD21 or WAPL depletion. Error bars indicate mean ± s.d. g, Dynamic model of how cohesin or cohesin-mediated structures may accelerate TF target search.

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