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. 2022 Dec 7;80(1):3. doi: 10.1007/s00018-022-04602-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Specification and location of hsa_circ_0000280. A Scheme of hsa_circ_0000280 production. B Specific divergent and convergent primers amplified hsa_circ_0000280 in cDNA and genomic DNA (gDNA) from HASMCs. C Circular junction site of hsa_circ_0000280 revealed by sequencing. D Validation of hsa_circ_0000280 Stability using RNase R and RT-PCR analysis (n = 3, p < 0.05; Student t test). E Fluorescence in situ hybridization assay (FISH) shows hsa_circ_0000280 localization in HASMCs. Control was18s rRNA. Probes for hsa_circ_0000280 and 18 s rRNA were labeled with Cy3. Scale bar, 20 µm. Data are presented as means ± SD