Fig. 7.

Neointimal hyperplasia is reduced by hsa_circ_0000280 in vivo. A Delivery of hsa_circ_0000280 into CCA completed. Level of hsa_circ_0000280 detected by qPCR (n = 6; p < 0.0001; Student t tests). B Hematoxylin–Eosin staining of intimal changes induced by ligation surgery after hsa_circ_0000280 delivery vs. E.vector. C Intimal/media thickness measurements (Scale bar, 100 µm; n = 6; p = 0.0008; two-way ANOVA, multiple t tests). D–G Representative immunohistochemical staining of SMA and CDKN1A (Scale bar, 100 µm; n = 5; p = 0.0444 vs. E.vector; p = 0.0079 compared with E.vector + ELAVL1^SMKO; p > 0.05; one-way ANOVA, multiple t tests). H, I Western blots of indicated proteins in CCA of four groups of mice (n = 6 per group; p < 0.05 vs. E.vector; p > 0.05 vs. E.vector + ELAVL1^SMKO; two-way ANOVA, multiple t tests). Data are presented as means ± SD