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. 2022 Dec 7;12:21123. doi: 10.1038/s41598-022-25684-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological phenotype of the Mpgcam1 Mprra double mutant. (a) Thalli of the Mpgcam1 and Mprra single mutants, and the Mpgcam1 Mprra double mutant were observed from the top (left), from the side (second from the right), and from the bottom (right). Magnified images of gemma cups or predicted position for gemma cup formation are shown (second from the left). The numbers in the side views indicate the number of curled thalli per total thalli. Bars represent 5 mm. (b) Gemma cup number per thallus area. Thallus apices were cultured for 14 days. Data are represented as mean ± SD (n = 20). Significant differences from the wild-type (Tak-1 and Tak-2) were determined by the Student’s t-test as follows: **P < 0.01.