Figure 5.

Morphological phenotype of the Mprrb knockout lines overexpressing MpGCAM1. (a) Transcript levels of MpGCAM1 in the wild-type (Tak-2) and Mprrb that overexpress MpGCAM1. Thalli were treated with 5 µM DEX for 14 days. mRNA levels were normalized to that of MpEF1α. Data are presented as mean ± SD (n = 3). Significant differences from the wild-type (Tak-2) were determined by the Student’s t-test as follows: **P < 0.01. (b) Thalli of the wild-type and Mprrb, which carry proMpEF1α:MpGCAM1–GR, were treated with (+ DEX) or without (mock) 5 µM DEX for 14 days. The DEX-treated thalli were transferred onto a medium without DEX and were further cultured for 28 days (+ DEX > >  − DEX). The lower panels for DEX-treated and recovered samples are magnified images of cell clumps in the tip region and regenerated thalli, respectively. Bars represent 2 mm.