Skip to main content
. 2022 Dec 7;13:7551. doi: 10.1038/s41467-022-35134-3

Fig. 3. Apc-mutant cells are sensitive to cell-intrinsic TGFβ signalling.

Fig. 3

a Quantification of BrdU-positive (left) and apoptotic cells (right) in VilCreER;Apcfl/fl;Alk5+/+ (blue) and VilCreER;Apcfl/fl;Alk5CA (orange) small intestinal crypts 3 and 4 days post-tamoxifen induction. n = 4 mice per group, except n = 3 mice per group for d3 BrdU data. Data were ±s.e.m; *P = 0.05, one-tail Mann–Whitney U-test. b Representative BrdU and H&E staining of small intestinal tissue from VilCreER;Apcfl/fl;Alk5+/+ and VilCreER;Apcfl/fl;Alk5CA mice 4 days post-tamoxifen induction. Scale bar, 100 μm. c Schematic illustrating the treatment timeline and tissue analysis from VilCreER;Apcfl/fl;Alk5CA mice. Tmx tamoxifen, ALK5i ALK5-inhibitor. d Representative IHC (p-SMAD3) and ISH (Alk5CA) staining on VilCreER;Apcfl/fl;Alk5CA mice following vehicle or ALK5i treatment. Scale bar, 200 μm. e Quantification of BrdU-positive (left) and apoptotic cells (right) from VilCreER;Apcfl/fl;Alk5CA mice following vehicle or ALK5i treatment. n = 3 mice per group, except for scoring of apoptotic bodies where n = 5 mice per group. Data were ±s.e.m. *P = 0.05, **P = 0.008; one-tail Mann–Whitney U-test for BrdU; two-tail Mann–Whitney U-test for apoptosis.