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. 2022 Dec 7;12:21193. doi: 10.1038/s41598-022-25576-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Percent survival of MDR P. aeruginosa infected G. mellonella larvae after phage vB_PaeS_VL1 treatment. Groups of 10 larvae were inoculated with either MDR P. aeruginosa strains (a) PA897, (b) PA294 or (c) PA269 follow by treatment with phage VL1 at MOI of 1,000 or 10,000 at 1 h p.i.. The numbers of dead larvae were determined every 12 h for 72 h p.i. GraphPad Prism software was used to graph and analyze the data using a Log-rank (Mantel-Cox) test. Asterisks indicated significant differences (*P < 0.05 or **P < 0.01) between bacterial infection groups, and the phage treatment groups. Data is representative of that obtained in three independent experiments (n = 3 biological replicates).