Table 1.
Host range infection and EOP of phage vB_PaeS_VL1 against 61 Pseudomonas and other bacteria. The lytic activity was determined by plaque assay. The results “ + ” indicates lytic or susceptibility; “ − ” indicates non − lytic or resistant; ND = Not determine.
| Strains | Drug Resistance | Source | Lytic activity | Efficiency of plating |
|---|---|---|---|---|
| P. aeruginosa ATCC27853 | AMP AMC CL FOX CPD SXT | Reference strain | + |
1.00 ± 0.00 (Propagation host) |
| PA898 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT AK CN | Ear swab/Cat | + |
0.61 ± 0.01 (High) |
| PA900 | AMP AMC CL FOX CRO CPD SXT TE DO OT | Nasal swab/Cat | − | ND |
| PA910 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Wound swab/Dog | + |
0.08 ± 0.01 (Low) |
| PA947 | AMP AMC CL FOX CRO CPD NOR CIP ENR SXT TE DO OT CN | Wound swab/Dog | + |
0.75 ± 0.04 (High) |
| PA881 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Wound swab/Dog | + |
0.08 ± 0.02 (Low) |
| PA1005 | AMP AMC CL FOX CRO CPD CTX NOR CIP ENR SXT TE DO OT CN | Wound swab/Cat | − | ND |
| PA1083 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Dog | + |
0.05 ± 0.02 (Low) |
| PA1093 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Abdominal/Dog | + |
0.88 ± 0.05 (High) |
| PA1017–61 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Cat | + |
0.85 ± 0.01 (High) |
| PA1170 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Cat | − | ND |
| PA1171 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Wound swab/Dog | + |
0.85 ± 0.05 (High) |
| PA729 | AMP AMC CL FOX CPD CTX SXT TE DO OT | Ear swab/Cat | + |
0.77 ± 0.01 (High) |
| PA708 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT AK CN | Ear swab/Dog | − | ND |
| PA819 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Urine/Cat | + |
0.84 ± 0.02 (High) |
| PA659–2 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Eye swab/Dog | + |
0.04 ± 0.01 (Low) |
| PA700 | AMP AMC CL FOX CPD CTX SXT TE DO OT | Wound swab/Dog | − | ND |
| PA814 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT | Wound swab/Dog | − | ND |
| PA801 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT CN | Wound swab/Dog | + |
0.68 ± 0.01 (High) |
| PA728 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT | Urine/Cat | + |
0.74 ± 0.03 (High) |
| PA722 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT | Urine/Dog | + |
0.79 ± 0.01 (High) |
| PA791 | AMP AMC CL FOX CRO CPD CAZ CTX SXT TE DO OT | Wound swab/Cat | − | ND |
| PA729 | AMP AMC CL FOX CPD CTX SXT TE DO OT | Ear sawb/Dog | + |
0.81 ± 0.03 (High) |
| PA723–2 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Dog | − | ND |
| PA755–2 | AMP AMC CL FOX CPD CTX SXT DO OT | Nasal swab/Cat | − | ND |
| PA392 | AMP AMC CL FOX CPD CTX SXT TE DO | Nasal swab/Cat | + |
0.78 ± 0.05 (High) |
| PA34 | AMP AMC CL FOX CRO CPD CAZ CTX NOR CIP ENR SXT TE DO OT CN | Urine/Dog | − | ND |
| PA294 | AMP AMC CL FOX CRO CPD CTX NOR CIP ENR SXT TE DO OT AK CN | Thoracic cavity/Cat | + |
0.45 ± 0.02 (Moderate) |
| PA149–4 | AMP AMC CL FOX CRO CPD CAZ CTX NOR CIP ENR SXT TE DO OT CN | Wound swab/Dog | − | ND |
| PA897 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT AK CN | Wound swab/Dog | + |
0.89 ± 0.03 (High) |
| PA41 | AMP AMC CL FOX CRO CPD CTX NOR CIP ENR SXT TE DO OT AK CN | Urine/Dog | − | ND |
| PA269 | AMP AMC CL FOX CRO CPD CAZ CTX NOR CIP ENR SXT TE DO OT AK CN | Urine/Dog | + |
0.05 ± 0.01 (Low) |
| PA276 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT CN | Urine/Dog | − | ND |
| PA212 | AMP AMC CL FOX CRO CPD CAZ CTX NOR SXT TE DO OT | Ear swab/Dog | − | ND |
| PA422 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Dog | − | ND |
| PA261 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Cat | + |
0.85 ± 0.03 (High) |
| PA280 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Dog | − | ND |
| PA187–2 | AMP AMC CL FOX CRO CPD CAZ CTX SXT TE DO | Wound swab/Dog | + |
0.77 ± 0.02 (High) |
| PA278 | AMP AMC CL FOX CRO CPD CAZ CTX SXT TE DO OT | Wound swab/Cat | − | ND |
| PA371–2 | AMP AMC CL FOX CRO CPD CAZ CTX NOR CIP ENR SXT TE DO OT AK CN | Wound swab/Dog | − | ND |
| PA1033 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Dog | + |
0.88 ± 0.02 (High) |
| PA1092 | AMP AMC CL FOX CPD MAR CTX SXT TE DO OT | Ear swab/Dog | + |
0.76 ± 0.04 (High) |
| PA1122 | AMP AMC CL FOX CRO CPD CTX NOR CIP ENR MAR SXT TE DO OT | Urine/Dog | + |
0.85 ± 0.02 (High) |
| PA1017–64 | AMP AMC CL FOX CPD SXT TE DO | Urine/Dog | + |
0.75 ± 0.05 (High) |
| PA1147 |
AMP AMC CL FOX CPD CTX SXT TE DO OT |
Urine/Cat | − | ND |
| PA1144 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Cat | − | ND |
| PA1030 | AMP AMC CL FOX CPD CTX NOR SXT TE DO OT | Ear swab/Dog | − | ND |
| PA970 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Dog | + |
0.87 ± 0.03 (High) |
| PA1069 | AMP AMC CL FOX CRO CPD CTX NOR CIP ENR MAR SXT TE DO OT CN | Wound swab/Dog | − | ND |
| PA703 | AMP AMC CL FOX CRO CPD CAZ ENR CTX SXT TE | Pus/Dog | + |
0.91 ± 0.03 (High) |
| PA720 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Urine/Cat | + | |
| PA286 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Wound swab/Dog | − | ND |
| PA284 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT AK CN | Urine/Dog | + |
0.37 ± 0.02 (Moderate) |
| PA270 | AMP AMC CL FOX CRO CPD CAZ CTX ENR SXT TE DO OT | Pus/Dog | + |
0.35 ± 0.06 (Moderate) |
| PA12 | AMP AMC CL FOX CRO CPD CTX SXT TE DO OT | Wound swab/Cat | + |
0.85 ± 0.01 (High) |
| PA15 | AMP AMC CL FOX CRO CPD NOR CIP ENR CTX SXT TE DO OT AK CN | Ear swab/ Cat | − | ND |
| PA53 | AMP AMC CL FOX CRO CPD CAZ ENR SXT TE DO OT | Abdominal fluid/ Cat | + |
0.61 ± 0.02 (High) |
| PA80 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Dog | − | ND |
| PA89 | AMP AMC CL FOX CRO CPD CAZ NOR CIP CTX SXT TE DO OT | Wound swab/Dog | + |
0.33 ± 0.02 (Moderate) |
| PA108 | AMP AMC CL FOX CRO CPD CTX ENR SXT TE DO OT | Wound swab/Cat | − | ND |
| PA112 | AMP AMC CL FOX CRO CPD NOR CIP ENR CTX SXT TE DO OT | Wound swab/Cat | + |
0.007 ± 0.01 (Low) |
|
Escherichia coli ATCC25922 |
ND | Reference strain | − | ND |
| Burkholderia thailandensis E264 | ND | Environmental sample | − | ND |
| Proteus mirabilis 22–65 | AMP SXT TE DO OT | Wound swab/Dog | − | ND |
|
Staphylococcus aureus ATCC25923 |
ND | Reference strain | − | ND |
|
Enterococcus faecalis ATCC29212 |
ND | Reference strain | − | ND |