Fig. 6. Local neural inputs are required for astrocyte regulation of lipolysis.

a–c Chemogenetic stimulation of astrocytes in the ARH with J60 via i.p. induced iWAT lipolysis in the astrocyte hM3Dq-transduced mice compared to tdTomato-transduced controls, as evidenced by increased levels of p-HSL and glycerol and plasma NEFA: a Representative western blots of p-HSL and HSL and actin in inguinal iWAT extracted 2 h post a single i.p. injection of J60 in astrocyte tdTomato or hM3Dq-transduced mice; b, c Group data of b iWAT glycerol level and c plasma NEFA (n = 6 per group). d–f LCFN and ACFN physical denervation blunted the astrocyte-stimulation-induced HSL phosphorylation: d, e Representative blots of p-HSL and HSL and actin in inguinal iWAT extracted 2 h post a single i.p. injection of J60, from astrocyte d hM3Dq- or e tdTomato-transduced mice which received a control Sham or crushing injury to iWAT-supporting LCFN and ACFN; and f Group data of p-HSL over HSL from astrocyte tdTomato or hM3Dq-transduced mice with sham or crushing surgeries respectively (Tdtomato^Sham, n = 4; Tdtomato^Den, n = 5; hM3Dq^Sham, n = 5; hM3Dq^Den, n = 5). Data represent mean ± s.e.m; two-tailed Student’s t-tests for b (p = 0.04) and c (p = 0.01); two-way ANOVA with Sidak post hoc tests for f (tdTomato^{Sham to Den}, p = 0.48; Sham^{tdTomato to hM3Dq}, p = 0.02; tdTomato^Sham to hM3Dq^Den, p = 0.99; tdTomato^Den to hM3Dq^Sham, p = 0.0004; tdTomato^Sham to hM3Dq^Den, p = 0.99; tdTomato^Den to hM3Dq^Den, p = 0.65; hM3Dq^{Sham to Den}, p = 0.008; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.