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. 2022 Dec 7;13:7536. doi: 10.1038/s41467-022-35258-6

Fig. 8. Inhibition of POMC neurons diminishes astrocytic elevation of iWAT NE contents.

Fig. 8

a Schematic illustration of targeting a vector carrying GfaABC1D-hM3Dq and a second vector carrying Cre-dependent hM4Di to the ARH and of implanting an FSCV carbon fiber electrode in iWAT in POMC-Cre mice. b NE probe calibration curve using increased volumes of NE (1 mM) following the detailed instructions for the probes. c Representative traces of real-time recording of the FSCV signals of iWAT in astrocyte hM3Dq POMC neuron mCherry (n = 5), astrocyte tdTomato POMC neuron hM4Di (n = 5), or astrocyte hM3Dq POMC neuron hM4Di (n = 6)-transduced mice, respectively. Mice were treated with J60 via i.p. after a stable baseline. d Averaged traces from c (AstrocytehM3Dq POMChM4Di, n = 6; AstrocytehM3Dq POMCmCherry, n = 5; AstrocytetdTomato POMChM4Di, n = 5). Two-way ANOVA with Sidak post hoc tests; data represent mean ± s.e.m.; **p = 0.006 < 0.01; ***p = 0.0002 < 0.001; ****p < 0.0001. Red arrows in b indicate the time points of adding NE in different volumes. Black arrows in d point to i.p. injections of J60.