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. 2000 Mar;68(3):1391–1399. doi: 10.1128/iai.68.3.1391-1399.2000

FIG. 2.

FIG. 2

FIG. 2

FIG. 2

FIG. 2

FIG. 2

The GPI anchor of DAF allows efficient internalization of purified Dr fimbriae and Dr+ E. coli through a glycolipid pathway. (A to D) Binding of purified Dr fimbriae (arrow) or latex beads coated with Dr fimbriae (arrow) to GPI-anchored DAF causes noncoated microinvaginations (arrowheads), leading to internalization. (E) Dr+ E. coli binds at comparable levels to the recombinant CHO cells expressing DAF-GPI and its chimeric TM anchor constructs. (F) Dr+ E. coli is taken up more efficiently into GPI-anchored DAF than into the TM fusion forms DAF/HLA-TM (P ≥ 0.10) and DAF/MCP-TM (P ≤ 0.004). Each bar represents the mean and standard error of the mean from 10 independent experiments, with each assay done in duplicate. (G) Endocytosis mediated by the GPI-anchored but not the TM version of DAF can be specifically inhibited by the sterol-chelating agent methyl-β-cyclodextrin, suggesting that the two pathways are distinct (∗, P ≤ 0.02). Error bars are the standard error of the mean from three independent experiments. Scale bars, 0.5 μm.