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Effect of proteinase K on immunoreactive Iha. OMPs of E. coli ORN172(pIha) (lanes 1 and 3), E. coli O157:H7 (lanes 2 and 4), E. coli ORN172(pSK+) (lanes 5 and 7), and E. coli O157:H7nalR(Δiha) (lanes 6 and 8) without (lanes 1, 2, 5, and 6) and with (lanes 3, 4, 7, and 8) proteinase K treatment, probed with α-Iha antibodies.
