TABLE 1.
Bacteria analyzed in this study
| Strain(s) | Reference or source | Homology
|
||
|---|---|---|---|---|
| ihab | TAIc | Ihad | ||
| E. coli O157:H7a | ||||
| 25 E. coli O157:H7 strains from Seattle, Australia, and Colombia, including strain 86-24 | 37, 5; M. Samadpour, S. Mattar, Australia Government Analytical Laboratories | 4.7, 7.5e | + | 67 |
| E. coli O157:H7 (86-24nalR) | 6 | 4.7 | + | NT |
| E. coli O157:H7 (86-24nalR) (Δiha) | This paper | 2.7f | + | ± |
| Sorbitol-fermenting E. coli O157:H− (493-89, 5412-89, CB569, 514-91) | 22, 43; H. Karch, L. Beutin | − | NT | |
| E. coli O55:H7 (5A-D, TB156A, TB182A) | 41, 7 | − | NT | |
| E. coli O55:H7 (5E) | 41) | 2.9 | V | − |
| eae+ STEC distantly related to E. coli O157:H7 | ||||
| E. coli O26:NM (TB352A) | 7 | 4.7, 10.1 | + | 39 |
| E. coli O85:NM (TB334C) | 7 | 4.7, 10.1 | + | − |
| E. coli O126:H2 (TB285A) | 7 | 4.7, 10.1 | + | − |
| E. coli O103:H2 (UTI) | 36 | 4.7 | + | − |
| E. coli O111:HN (TB226A) | 7 | 4.7 | + | − |
| E. coli O103:H6 (TB154A) | 7 | − | NT | |
| eae-negative STEC distantly related to E. coli O157:H7 | ||||
| E. coli O104:H21 | 3; T. Damrow | 3.9, 10.1 | V | 56 |
| E. coli O113:H21 (CL15) | 11 | 7.9 | V | 56 |
| stx-negative EPEC distantly related to E. coli O157:H7 | ||||
| E. coli O15:NM (RDEC-1) | 8 | 4.7 | + | − |
| E. coli O111:NM (B171) | 32 | − | NT | |
| E. coli O119:H6 (659-79) | 23 | − | NT | |
| E. coli O127:H6 (E2348/69) | 23 | − | NT | |
| E. coli O142:H6 (E851/71) | 23 | − | NT | |
| E. coli O111:H− (2430-78) | 23 | 5.1 | + | − |
| Other | ||||
| E. coli ORN172(pSK+) | 42, this paper | − | − | |
| E. coli ORN172(pIHA) | 42, this paper | 2.2g | − | 78 |
| 20 E. coli strains from nondiarrheal human stools | S. Moseley | 3.6h | NT | NT |
Including derivatives and closely related strains.
Size (in kilobases) of BstXI fragment(s) detected by iha probe (the insert of pIha). −, iha homologue was not detected with this probe.
Characterization of BstXI fragments detected by TAI probe. +, TAI homologue detected. −, TAI probe detects BstXI fragments that are of different sizes, and fewer, than those detected in E. coli O157:H7; homology is considerably less intense, and the iha homologue is absent. V, one or more BstXI framgents are intense on a Southern blot using the TAI probe, but the pattern of bands detected by the TAI probe is different from that of TAI in E. coli O157:H7; iha homologue(s) is present. NT, not tested.
Size (in kilodaltons) of the largest OMP detected by α-Iha antibodies. NT, not tested. −, antigen not detected; ±, faint 67-kDa antigen detected in some OMP preparations.
In five E. coli O157:H7 strains, a 7.5-kb BstXI fragment is also detected by the iha probe.
Faint 2.7-kb band detected, representing homology of iha probe to intact 5′ and 3′ ends of truncated iha gene.
iha is cloned into pSK+ in this laboratory strain. Size reflects insert length, not BstXI fragment.
Only three of these isolates contain iha homologues.