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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Struct Biol. 2022 Oct 1;214(4):107901. doi: 10.1016/j.jsb.2022.107901

Figure 3:

Figure 3:

Simulation of optically driven sample heating. a) Simulation geometry intended to represent a single grid square. The boundaries of the grid square were fixed to be 77 K and sample heating was due to the absorption of a 561 nm laser with a Gaussian beam profile. The amount of energy absorbed and how it was dissipated depended on the various support film material properties. b) Representative isotherms from solving equation 1 for the standard carbon grid illuminated by a Gaussian beam with a peak intensity of 50 W/cm2. The red star marks the peak temperature, in this case ~120 K c) Plot of the peak temperature as a function of excitation intensity for the three different support films. The red star is the same as that shown in b.