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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Arch Oral Biol. 2022 Nov 9;145:105582. doi: 10.1016/j.archoralbio.2022.105582

Fig. 1. Schematic study design.

Fig. 1.

We used a duo species biofilm model to evaluate the effect of antifungal medications on S. mutans and C. albicans duo-species biofilm formation. Culture medium was changed once/daily at 20 and 44 hours (marked as green dot). Biofilms grew under 0.1% sucrose condition in the first 20hours, then were subjected to sugar challenge with 1% sucrose and 1% glucose. Nystatin (treatment group) and PBS (control group) applications were administered 4 times/day, 5 minutes/application, between 20–68 hours (marker in purple).