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The real-time PCR was performed to assess C. albicans virulence genes. CHT2, HWP1, ECE1, and ERG4 were significantly down-regulated (p<0.05, t-test) immediately following the first Nystatin application at 24h. SOD3 gene was significantly down-regulated (p<0.05, t-test) after 30 hours. At 44 hours which is after 4 applications of Nystatin, the expressions of all C. albicans virulence genes were not detectable.
