Table 3.
Non-lineage-defining mutations in the spike protein found in this study.
| Mutation | Na | Frequency (%) | N (%) in non-VOC | N (%) in Alpha | N (%) in Gamma | N (%) in Delta |
|---|---|---|---|---|---|---|
| L5F | 8 | 3.6% | 0 (0%) | 8 (7%) | 0 (0%) | 0 (0%) |
| S12F | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| L18F | 1 | 0.4% | 0 (0%) | 1 (1%) | NAb | 0 (0%) |
| R21T | 24 | 10.7% | 0 (0%) | 1 (1%) | 0 (0%) | 23 (22%) |
| T22I | 1 | 0.4% | 1 (25%) | 0 (0%) | 0 (0%) | 0 (0%) |
| T29I | 2 | 0.9% | 2 (50%) | 0 (0%) | 0 (0%) | 0 (0%) |
| T29A | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| A67V | 7 | 3.1% | 0 (0%) | 0 (0%) | 0 (0%) | 7 (7%) |
| T95I | 11 | 4.9% | 0 (0%) | 0 (0%) | 0 (0%) | 11 (10%) |
| K97Q | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| K147N | 3 | 1.3% | 0 (0%) | 3 (3%) | 0 (0%) | 0 (0%) |
| K150E | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| M153I | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| S221L | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| A222V | 64 | 28.4% | 1 (25%) | 0 (0%) | 0 (0%) | 63 (59%) |
| T250S | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| T250I | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| P251L | 2 | 0.9% | 0 (0%) | 0 (0%) | 0 (0%) | 2 (2%) |
| A352S | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| N439K | 1 | 0.4% | 1 (25%) | 0 (0%) | 0 (0%) | 0 (0%) |
| K444N | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| P479S | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| A522S | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| E583D | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| S640F | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| A653V | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| A688V | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| T719I | 3 | 1.3% | 0 (0%) | 0 (0%) | 0 (0%) | 3 (3%) |
| K795R | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| P812L | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| D843N | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| D936H | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| V976F | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| A1056V | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| Q1071L | 2 | 0.9% | 2 (50%) | 0 (0%) | 0 (0%) | 0 (0%) |
| G1085R | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| I1115V | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| S1147L | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| T1160I | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| G1219V | 3 | 1.3% | 0 (0%) | 3 (3%) | 0 (0%) | 0 (0%) |
| V1228L | 1 | 0.4% | 0 (0%) | 1 (1%) | 0 (0%) | 0 (0%) |
| I1232L | 4 | 1.8% | 0 (0%) | 4 (4%) | 0 (0%) | 0 (0%) |
| M1237V | 1 | 0.4% | 0 (0%) | 0 (0%) | 0 (0%) | 1 (1%) |
| V1264L | 2 | 0.9% | 0 (0%) | 1 (1%) | 0 (0%) | 1 (1%) |
| L1265I | 8 | 3.5% | 0 (0%) | 0 (0%) | 0 (0%) | 8 (8%) |
a
N = 225, consisting of 4 non-VOC, 112 Alpha, 3 Gamma, and 106 Delta isolates.
b
Not applicable, this mutation is a defining mutation of the Gamma variant.