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. 2022 Nov 24;13:1052091. doi: 10.3389/fphar.2022.1052091

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Copyright © 2022 Roefs, Heusermann, Brans, Snijders Blok, Lei, Vader and Sluijter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of PalmGFP- and PalmtdTomato-labeled EVs derived from CPCs. (A) Fluorescent microscopy pictures of CPC cells stably expressing (top) PalmGFP or (bottom) PalmtdTomato. Scale bars represent 400 µm. (B) Representative NTA plots showing the size distribution and particle concentration of EVs after SEC isolation of conditioned medium derived from PalmGFP⁺- and PalmtdTomato⁺ CPCs. (C) Western blot analysis showing the presence of CD81, Syntenin-1 (SYNT), AnnexinA1 (ANXA1), GAPDH, PalmGFP and/or PalmtdTomato, and absence of Calnexin (CNX) in (left) PalmGFP⁺- and (right) PalmtdTomato⁺ EVs. Cell lysates (CL) derived from (left) PalmGFP⁺ or (right) (TdTomato-negative) CPCs were included as control. Uncut blots are included in Supplementary Figure S10. (D) Representative TEM images of PalmGFP⁺ EVs at two different magnifications.