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. 2022 Nov 24;13:1052091. doi: 10.3389/fphar.2022.1052091

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Copyright © 2022 Roefs, Heusermann, Brans, Snijders Blok, Lei, Vader and Sluijter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunocytochemistry analysis of EV uptake in the heart. PalmtdTomato-EVs were administered in the left ventricle wall of a healthy mouse heart through intramyocardial injection and heart tissue was collected after 4 h (A–F) Immunofluorescence staining of two subsequent heart sections, cut across the sagittal plane, using antibodies against tdTomato (shown in red), and co-staining with antibodies against (A–C) cardiomyocyte marker cardiac Troponin I (cTnI, shown in green) and (D–F) blood vessel-specific CD31 (shown in green). (B) Enlargement of the square in panel A. (C) Enlargement of the square in panel B. (E) Enlargement of the square in panel D. (F) Enlargement of the square in panel E. Nuclei are visualized with DAPI (shown in blue). tdTomato co-localization with other stainings are indicated with arrows: cTnI (white), CD31 (yellow), no co-localization (red). Scale bars = 50 μm (A,D), 250 μm (B,E), 100 μm (C,F).