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. 2022 Nov 24;9:1023438. doi: 10.3389/fcvm.2022.1023438

Figure 1.

Figure 1

EEEG treatment reduces macrophage foam cell formation. (A) Effect of EEEG treatment on BMDM viability. (B) Foam cell formation assayed by oil-red-O staining. Cells were incubated for 24 h with ox-LDL in the presence or absence of EEEG, followed by Oil red O staining. (C) Quantification of ox-LDL uptake by macrophages. (D) BODIPY staining of RAW 264.7 cells. Cells were incubated for 24 h with ox-LDL in the presence or absence of EEEG, followed by BODIPY staining. (E) Quantification of BODIPY fluorescence staining. Data are expressed as mean ± s.e.m., Statistical analysis was based on Graphpad Prism 9.0.2 software and a value of P < 0.05 was considered statistically significant. One-way ANOVA with Student Neuman-Keuls post-hoc test was performed to compare the data between multiple groups, *p < 0.05, ****p < 0.0001 vs. ox-LDL.