Figure 6.

EEEG treatment significantly inhibits macrophage infiltration in the aortic root of mice. (A) Representative images of CD68 (macrophages, red) and α-SMA (α-smooth muscle actin; green, smooth muscle cells) immunostaining of aortic root sections. (B,C) Quantitative analysis of macrophages and smooth muscle cell content in the aortic root sections. HFD (n = 5), HFD+1 g/kg EEEG (n = 5), HFD+2 g/kg EEEG (n = 5), HFD+4 g/kg EEEG (n = 5), Data are expressed as mean±s.e.m., Statistical analysis was based on Graphpad Prism 9.0.2 software and a value of P < 0.05 was considered statistically significant. One-way ANOVA with Student Neuman-Keuls post-hoc test was performed to compare the data between multiple groups, *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significance.