REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Ki67 antibody	Abcam	Cat# ab15580; RRID:AB_443209
Alpha-Tubulin antibody	Sigma-Aldrich	T9026; RRID:AB_477593
RSK-1 antibody	CST	#8408; RRID:AB_10828594
P-RSK antibody	CST	#8753; RRID:AB_2783561
P-RSK antibody	CST	#9344; RRID:AB_331650
GAPDH antibody	CST	#5174; RRID:AB_10622025
Vinculin antibody	Sigma-Aldrich	V9131; RRID:AB_477629
Biological samples
Patient Derived Xenografts	Dr. Manuel Hidalgo, Spanish National Cancer Research Center (CNIO), Madrid, Spain	(Reference no. I409181220BSMH)
Chemicals, peptides, and recombinant proteins
LY3214996 (Temuterkib)	Lilly	Available at https://www.selleckchem.com/ Catalog No.S8534
RMC-4550	Revolution Medicines	Available at https://www.selleckchem.com/ Catalog No.S8718
37% Formaldehyde solution	Merck	104,003
Crystal Violet	Sigma-Aldrich	HT90132-1 L
Matrigel	Corning	REF 354230 LOT 9364004
Critical commercial assays
CellTiter-Glo 3D	Promega	REF G9682 LOT 0000496328
Caspase 3/7 reagent	Sartorius	#4440
Deposited data
Uncropped Western Blots	Mendeley	Mendeley Data: https://doi.org/10.17632/cz4vfsk28h.1
RNAseq Data	GEO	GEO: GSE213706
Experimental models: Cell lines
Murine KCP cell lines derived from KCP mice in house	This paper	N/A
MiaPaCa-2	ATCC	CRL-1420; RRID: CVCL_0428
ASPC1	ATCC	CRL-1682; RRID: CVCL_0152
Panc10.05	ATCC	CRL-2547; RRID: CVCL_1639
Panc-1	ATCC	CRL-1469; RRID: CVCL_1794
YAPC	DSMZ	REF 15070; RRID: CVCL_0480
Experimental models: Organisms/strains
B6 (C57BL/6J) mice	Jackson Laboratory	RRID:IMSR_JAX:000,664
NSG (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) mice	Jackson Laboratory	RRID:IMSR_JAX:005,557
KCP mice (Krastm4Tyj); (Ptf1atm1(Cre)Hnak); (Trp53tm1Brn) (KCP)	See References59,60,61	N/A
KCPmut mice (Krastm4Tyj); (Tg(Pdx1-cre)6Tuv); Trp53tm1Tyj)	See Reference62	N/A
NU-Foxn1nu nude mice (Hsd:Athymic Nude-Foxn1nu/Foxn1+)	Envigo https://www.envigo.com/model/hsd-athymic-nude-foxn1nu-foxn1?selctry=France&ctry=	N/A
Software and algorithms
ImageJ	https://imagej.nih.gov/ij/	RRID:SCR_003070
CompuSyn	https://www.combosyn.com/ See Reference63	N/A
Adobe Illustrator®	Adobe https://www.adobe.com/de/	RRID:SCR_010279
GraphPad Prism 8	GraphStats Technologies https://www.graphstats.net/	RRID:SCR_002798
Nucline™ NanoScan Software	Mediso	N/A
Flywheel DICOM viewer	Flywheel https://docs.flywheel.io/hc/en-us	N/A
Incucyte ZOOM	Essen Bioscience	N/A
Aperio ImageScope	Leica Biosystems https://www2.leicabiosystems.com/
Axiovision	Zeiss https://www.micro-shop.zeiss.com/de/de/system/software-axiovision+software-produkte/1007/
BioRender	https://biorender.com/