Figure 5.

Transcriptomic analysis reveals that NKp46⁺NK1.1⁺ subsets from the LP have unique features

(A) PCA of 15,658 NKp46⁺NK1.1⁺ cells from the LP.

(B) Heatmap showing the top 15 genes with the lowest or highest PC1 score, ranked according to their score value.

(C) UMAP projection of NKp46⁺NK1.1⁺ cells from the LP clustered on the basis of RNA levels. The donut graph shows the percentage of each LP NKp46⁺NK1.1⁺ subset identified.

(D) Heatmap of the top 10 upregulated DEGs of the identified NKp46⁺NK1.1⁺ clusters, ranked by adjusted p value (FC > 0.35 and p < 0.05).

(E) Violin plots showing mRNA expression profiles of selected genes across Seurat clusters.

(F and G) Pseudotime analysis of cells included in NK (F) and ILC1 (G) clusters. UMAPs are colored according to pseudotime scores (left) and cell cluster (right).

(H and I) Normalized expression of genes along the pseudotime axis calculated for the cells included in NK (H) and ILC1 (I) clusters. Color bars indicate cluster identity.

(J) UMAP plot overlaid with tissue-resident and circulating lymphocyte signatures.

(K) Module score analysis for tissue-resident and circulating lymphocyte signatures for clusters identified in (C) (mean ± SD).