Skip to main content
. 2022 Oct 20;3(11):100792. doi: 10.1016/j.xcrm.2022.100792

Figure 4.

Figure 4

AAV8-Lhcgr promotes the formation of elongating spermatids

(A) Representative GO terms of the top 500 upregulated genes in male Lhcgr−/− mice treated with AAV8-Lhcgr versus PBS (n = 3).

(B) Heatmap showing the expression of marker genes for the four major germ cell types in the testicular transcriptional profiles.

(C–F) Representative images of testis sections from the four groups (n = 4). Sections were immunostained for PNA (C), and DDX4 and TNP2 (E), and counterstained with DAPI. Quantitative analysis showing the percentage of PNA+ (D) and TNP2+ (F) germ cells in the seminiferous tubules of the testes. NC, negative control. Scale bar: 50 μm. Data are represented by boxplots, and whiskers show the minimum to maximum values. ∗p < 0.05; ∗∗∗p < 0.001; ns, not significant.