REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
CD14-FITC (RMO52 clone) | Beckman Coulter | Cat# IM0645U; RRID: AB_130992 |
CD45-PC5 (J33 clone) | Beckman Coulter | Cat# IM2653U; RRID: AB_10641226 |
HLA-DR-RD1 (9-49 clone) | Beckman Coulter | Cat# 6604366; RRID: AB_2832962 |
IgG1-PE isotype control (679.1Mc7 clone) | Beckman Coulter | Cat# A07796; RRID: AB_2832963 |
IgG1-FITC isotype control (679.1Mc7 clone) | Beckman Coulter | Cat# A07795; RRID: AB_2832964 |
Quantibrite Anti-HLA-DR/Anti-Monocyte (L243/MφP9) | BD Biosciences | Cat# 340827; RRID: AB_400137 |
Mouse Anti-Human IL-6-PE (AS12 clone) | BD Biosciences | Cat# 340527; RRID: AB_400442 |
Chemicals, peptides, and recombinant proteins | ||
VersaLyse Lysing Solution | Beckman Coulter | A09777 |
Fixative Solution | Beckman Coulter | A07800 |
RPMI 1640 W/Stable Glutamine W/25 MM HEPES | SigmaAldrich | R0833 |
Lymphosep, Lymphocyte Separation Media | Biowest | L0560 |
PBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium | Biowest | L0615 |
FBS Superior; standardized Fetal Bovine Serum, EU-approved | Biochrom | S0615 |
Gentamycin Sulfate BioChemica | PanReac AppliChem | A1492 |
Penicillin G Potassium Salt BioChemica | PanReac AppliChem | A1837 |
Lipopolysaccharides from Escherichia coli O 55:B5 | Sigma-Aldrich | L2880 |
Phytohaemagglutinin from Phaseolus vulgaris | Roche | SKU 11249738001 |
Critical commercial assays | ||
Human TNFa uncoated ELISA | Invitrogen | 88–7346 |
Human Ferritin ELISA | ORGENTEC Diagnostika GmbH | ORG 5FE |
Software and algorithms | ||
SPSS | IBM | https://www.ibm.com/analytics/spss-statistics-software |
Other | ||
Kineret | Swedish Orphan Biovitrum | https://www.ema.europa.eu/en/documents/product-information/kineret-epar-product-information_en.pdf |
Immukine | Boehringer Ingelheim | https://www.ema.europa.eu/en/documents/psusa/interferon-gamma-list-nationally-authorised-medicinal-products-psusa/00001760/201601_en.pdf |