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. 2022 Nov 15;3(11):100817. doi: 10.1016/j.xcrm.2022.100817
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CD14-FITC (RMO52 clone) Beckman Coulter Cat# IM0645U; RRID: AB_130992
CD45-PC5 (J33 clone) Beckman Coulter Cat# IM2653U; RRID: AB_10641226
HLA-DR-RD1 (9-49 clone) Beckman Coulter Cat# 6604366;
RRID: AB_2832962
IgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat# A07796;
RRID: AB_2832963
IgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat# A07795;
RRID: AB_2832964
Quantibrite Anti-HLA-DR/Anti-Monocyte (L243/MφP9) BD Biosciences Cat# 340827;
RRID: AB_400137
Mouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat# 340527;
RRID: AB_400442

Chemicals, peptides, and recombinant proteins

VersaLyse Lysing Solution Beckman Coulter A09777
Fixative Solution Beckman Coulter A07800
RPMI 1640 W/Stable Glutamine W/25 MM HEPES SigmaAldrich R0833
Lymphosep, Lymphocyte Separation Media Biowest L0560
PBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615
FBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615
Gentamycin Sulfate BioChemica PanReac AppliChem A1492
Penicillin G Potassium Salt BioChemica PanReac AppliChem A1837
Lipopolysaccharides from Escherichia coli O 55:B5 Sigma-Aldrich L2880
Phytohaemagglutinin from Phaseolus vulgaris Roche SKU 11249738001

Critical commercial assays

Human TNFa uncoated ELISA Invitrogen 88–7346
Human Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE

Software and algorithms

SPSS IBM https://www.ibm.com/analytics/spss-statistics-software

Other

Kineret Swedish Orphan Biovitrum https://www.ema.europa.eu/en/documents/product-information/kineret-epar-product-information_en.pdf
Immukine Boehringer Ingelheim https://www.ema.europa.eu/en/documents/psusa/interferon-gamma-list-nationally-authorised-medicinal-products-psusa/00001760/201601_en.pdf