Figure 2.

Fc glycosylation of HLA-A2-specific antibodies

(A and B) Representative extracted ion chromatograms and mass spectra illustrating the observed variability between HLA-A2-specific (A) and total (B) IgG1 Fc glycosylation patterns of the same patient.

(C) Volcano plot displaying the log10-fold change (x axis) and −log10 p value (y axis) of individual IgG1 (blue circle) and IgG2/3 (maroon square) glycoforms between total and HLA-A2-specific IgG1 and IgG2/3, respectively.

(D) Violin plots showing the relative prevalence of glycans on bulk and HLA-A2-specific IgG1 (top) and IgG2/3 (bottom), respectively.

Statistical analysis was performed using a paired two-tailed Student’s t test (∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001, respectively).