REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse Anti-Human IgG Fc-PE (JDC-10)	SouthernBiotech	Cat# 9040-09; RRID:AB_2796601
PE Goat anti-mouse IgG Antibody (Clone Poly4053)	Biolegend	Cat# 405307; RRID:AB_315010
Mouse Anti-Human IgG1 Fc-PE (HP6001)	SouthernBiotech	Cat# 9054-09; RRID:AB_2796628
Mouse Anti-Human IgG2 Fc-PE (HP6002)	SouthernBiotech	Cat# 9070-09; RRID:AB_2796639
Goat Anti-Mouse IgG3, Human ads-AP	SouthernBiotech	Cat# 1100-09; RRID:AB_2794576
Mouse Anti-Human IgG4 Fc-PE (HP6025)	SouthernBiotech	Cat# 9200-09; RRID:AB_2796693
Biological samples
Human serum from anti-A2 sensitized patients	Hôpital Erasme ULB, Brussels, Belgium	N/A
Polyclonal immunoglobulin G (from Normal Human Plasma)	Athens Research & Technology	Cat#16-16-090707
Chemicals, peptides, and recombinant proteins
Protein A
Protein G
2-Deoxy-2-fluoro-L-fucose	Carbosynth	Cat# MD06089
HLA-A2	NIH Tetramer Facility
HLA-A1	NIH Tetramer Facility
HSV-gD (Ectodomain)	Immune Technology	Cat# IT-005-055p
Albumin, Bovine Fraction V (BSA)	RPI	Cat# A30075-250.0
Tween 20	Sigma Aldrich	Cat# P9416-100ML
Streptavidin Magnetic Beads	NEB	Cat# S1420S
Streptavidin-R-Phycoerythrin	Agilent	Cat# PJ31S- 1
NeutrAvidin	ThermoFisher Scientific	Cat# 31000
QUANTI-Luc	Invivogen	Cat# rep-qlc2
eBioscience™ Cell Stimulation Cocktail (500X)	ThermoFisher Scientific	Cat# 00-4970-93
LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation	ThermoFisher Scientific	Cat# L34964
Electron Microscopy Sciences 16% Paraformaldehyde Aqueous Solution, EM Grade, Bottle 100 ML	Fisher Scientific	Cat# 15710
FcγRIIIa V158	In house (Boesch et al. 2014)104	N/A
FcγRI	In house (Boesch et al. 2014)104	N/A
Melon™ Gel IgG Spin Purification Kit	ThermoFisher Scientific	Cat# 45206
Chromabond C18ec beads	Macherey-Nagel	Cat#30611
Sequencing Grade Modified Trypsin	Promega	Cat#V511A
Protein G Sepharose® 4 Fast Flow beads	GE Healthcare	Cat#17-0618-05
Formic Acid	Sigma-Aldrich	Cat#94318; CAS: 64-18-6
Acetonitrile (LC-MS grade)	Biosolve	Cat#012078; CAS: 75-05-8
Trifluoroacetic Acid (LC-MS grade)	Merck	Cat#85183; CAS: 76-05-1
Ammonium Bicarbonate	Sigma-Aldrich	Cat#09830; CAS: 1066-33-7
Critical commercial assays
QUANTI-Luc Lucia luciferase reporter gene system	invivogen	rep-qlc2
RNeasy kit	Qiagen	Cat# 74104
Deposited data
Liquid chromatography - Mass spectrometry data	This paper	https://doi.org/10.25345/C5Q52FJ02 (Accession code: MSV000090430)
Experimental models: Cell lines
Mouse HLA-A2 hybridoma cells	ATCC	HB-82 BB7.2
HEK-expi293 cells
Jurkat Lucia NFAT-CD16 cells	Invivogen	Cat# jktl-nfat-cd16
A375 cells	ATCC	CRL-1619
NK-92 human NK cells	NantKwest/ImmunityBio	https://immunitybio.com/
Recombinant DNA
pCMV-M1	Addgene	Cat# 23007
Software and algorithms
Flowjo v10	Flowjo	https://www.flowjo.com/
Prism	GraphPad	https://www.graphpad.com/
Bruker Compass DataAnalysis	Bruker Daltonics	https://www.bruker.com
MSConvertGUI	ProteoWizard	https://proteowizard.sourceforge.io
LaCyTools	LaCyTools (Jansen et al. 2016)105	https://git.lumc.nl/cpm/lacytools
BioRender	BioRender	https://biorender.com
Microsoft Office Excel and PowerPoint	Microsoft	https://microsoft.com
Other
96-well EIA/RIA clear flat bottom polystyrene high bind microplate	Corning	Cat# 3361
96 well clear bottom sterile tissue culture treated plates with lid	Thomas Scientific	Cat# 6916A05
Suspension culture microplate, 96 well, PS, U-bottom, with lid, sterile, single packed	Grenier bio-one	Cat# 650185
USA Scientific Inc 96-well PlateOne PP V-bottom plate, non-sterile	Fisher Scientific	Cat# 18339600
Octet® High Precision Streptavidin 2.0 (SAX2) Biosensors	Sartorius	Cat# 18-5136